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Pfizer Inc mason pfizer monkey virus
Mason Pfizer Monkey Virus, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mason-pfizer+monkey+virus/mason+monkey+pfizer+virus/pm42278261-146-26-26
Average 86 stars, based on 1 article reviews
mason pfizer monkey virus - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Retroviral:

Article Title: Rev-RRE activity modulates HIV-1 replication and latency reactivation: Implications for viral persistence and cure strategies
Article Snippet: “Simple” retroviruses, such as the Mason-Pfizer Monkey Virus, contain an RNA structure in the IR-mRNAs, the CTE [ ], that directly recruits the cellular factors Nxf1 and Nxt1 to permit nuclear export.

Article Title: Unveiling the DHX15-G-patch interplay in retroviral RNA packaging.
Article Snippet: This study reveals a broad aspect of retroviral replication and highlights the critical role of the cellular DEAH box RNA helicase DHX15 in the life cycle of Mason-Pfizer monkey virus.

Article Title: Unveiling the DHX15-G-patch interplay in retroviral RNA packaging.
Article Snippet: Interestingly, Mason-Pfizer monkey virus (M-PMV), a member of the betaretroviral family, is unique among viruses by encoding a G-patch motif ( 18 ).

Article Title: DHX15 inhibits mouse APOBEC3 deamination
Article Snippet: It has recently been shown to play a role in viral genome packaging of Mason-Pfizer monkey virus through interaction with a glycine-rich region (G-patch) in Gag that is unique to this virus [ ].

Article Title: The snakehead retrovirus promoter functions independently of the 3'ORF protein and its products are maternally inherited in transgenic zebrafish.
Article Snippet: Examples include the constitutive transport element (CTE) of the Mason-Pfizer monkey virus (MPMV), which binds the NXF1(TAP)/ NXT1 cellular factors to enhance nuclear export of unspliced viral RNAs [32,33], or the 115-nucleotide direct repeat (DR) that flanks the v-src gene in the Rous sarcoma virus.

Article Title: Determinants in the HTLV-1 Capsid Major Homology Region that are Critical for Virus Particle Assembly.
Article Snippet: The MHR region has been extensively studied among other different retroviruses, including HIV-1 [23-32], Rous sarcoma virus (RSV) [32-35], Mason-Pfizer monkey virus (MPMV) [36], and murine leukemia virus (MLV) [37].

Article Title: DHX15 inhibits mouse APOBEC3 deamination.
Article Snippet: It has recently been shown to play a role in viral genome packaging of Mason-Pfizer monkey https://doi.org/10.1371/journal.ppat.1013045.g004 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013045 April 1, 2025 10 / 18 virus through interaction with a glycine-rich region (G-patch) in Gag that is unique to this virus [56].

Article Title: Allostery and inter-domain dynamics in NXF1: An insight into viral CTE-RNA binding.
Article Snippet: Nucleocytoplasmic export of mRNA is a fundamental process in eukaryotic cells, facilitating the transportation of mRNA transcripts from nucleus to cytoplasm.. Central to this pathway is Nuclear Export Factor 1 (NXF1), a key RNA binding protein (RBP) mediating mRNA export through the Nuclear Pore Complex (NPC).. The significance of NXF1 in the export pathway extends to viral infections and neurodegenerative diseases where aberrations in nucleocytoplasmic transport have been identified as critical factors in disease progression.

Virus:

Article Title: Rev-RRE activity modulates HIV-1 replication and latency reactivation: Implications for viral persistence and cure strategies
Article Snippet: “Simple” retroviruses, such as the Mason-Pfizer Monkey Virus, contain an RNA structure in the IR-mRNAs, the CTE [ ], that directly recruits the cellular factors Nxf1 and Nxt1 to permit nuclear export.

Article Title: Unveiling the DHX15-G-patch interplay in retroviral RNA packaging.
Article Snippet: This study reveals a broad aspect of retroviral replication and highlights the critical role of the cellular DEAH box RNA helicase DHX15 in the life cycle of Mason-Pfizer monkey virus.

Article Title: Unveiling the DHX15-G-patch interplay in retroviral RNA packaging.
Article Snippet: Interestingly, Mason-Pfizer monkey virus (M-PMV), a member of the betaretroviral family, is unique among viruses by encoding a G-patch motif ( 18 ).

Article Title: DHX15 inhibits mouse APOBEC3 deamination
Article Snippet: It has recently been shown to play a role in viral genome packaging of Mason-Pfizer monkey virus through interaction with a glycine-rich region (G-patch) in Gag that is unique to this virus [ ].

Article Title: The snakehead retrovirus promoter functions independently of the 3'ORF protein and its products are maternally inherited in transgenic zebrafish.
Article Snippet: Examples include the constitutive transport element (CTE) of the Mason-Pfizer monkey virus (MPMV), which binds the NXF1(TAP)/ NXT1 cellular factors to enhance nuclear export of unspliced viral RNAs [32,33], or the 115-nucleotide direct repeat (DR) that flanks the v-src gene in the Rous sarcoma virus.

Article Title: Determinants in the HTLV-1 Capsid Major Homology Region that are Critical for Virus Particle Assembly.
Article Snippet: The MHR region has been extensively studied among other different retroviruses, including HIV-1 [23-32], Rous sarcoma virus (RSV) [32-35], Mason-Pfizer monkey virus (MPMV) [36], and murine leukemia virus (MLV) [37].

Article Title: DHX15 inhibits mouse APOBEC3 deamination.
Article Snippet: It has recently been shown to play a role in viral genome packaging of Mason-Pfizer monkey https://doi.org/10.1371/journal.ppat.1013045.g004 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013045 April 1, 2025 10 / 18 virus through interaction with a glycine-rich region (G-patch) in Gag that is unique to this virus [56].

Article Title: Allostery and inter-domain dynamics in NXF1: An insight into viral CTE-RNA binding.
Article Snippet: Nucleocytoplasmic export of mRNA is a fundamental process in eukaryotic cells, facilitating the transportation of mRNA transcripts from nucleus to cytoplasm.. Central to this pathway is Nuclear Export Factor 1 (NXF1), a key RNA binding protein (RBP) mediating mRNA export through the Nuclear Pore Complex (NPC).. The significance of NXF1 in the export pathway extends to viral infections and neurodegenerative diseases where aberrations in nucleocytoplasmic transport have been identified as critical factors in disease progression.

Infection:

Article Title: Rev-RRE activity modulates HIV-1 replication and latency reactivation: Implications for viral persistence and cure strategies
Article Snippet: “Simple” retroviruses, such as the Mason-Pfizer Monkey Virus, contain an RNA structure in the IR-mRNAs, the CTE [ ], that directly recruits the cellular factors Nxf1 and Nxt1 to permit nuclear export.

Article Title: Unveiling the DHX15-G-patch interplay in retroviral RNA packaging.
Article Snippet: This study reveals a broad aspect of retroviral replication and highlights the critical role of the cellular DEAH box RNA helicase DHX15 in the life cycle of Mason-Pfizer monkey virus.

Article Title: Unveiling the DHX15-G-patch interplay in retroviral RNA packaging.
Article Snippet: Interestingly, Mason-Pfizer monkey virus (M-PMV), a member of the betaretroviral family, is unique among viruses by encoding a G-patch motif ( 18 ).

Article Title: DHX15 inhibits mouse APOBEC3 deamination
Article Snippet: It has recently been shown to play a role in viral genome packaging of Mason-Pfizer monkey virus through interaction with a glycine-rich region (G-patch) in Gag that is unique to this virus [ ].

Article Title: The snakehead retrovirus promoter functions independently of the 3'ORF protein and its products are maternally inherited in transgenic zebrafish.
Article Snippet: Examples include the constitutive transport element (CTE) of the Mason-Pfizer monkey virus (MPMV), which binds the NXF1(TAP)/ NXT1 cellular factors to enhance nuclear export of unspliced viral RNAs [32,33], or the 115-nucleotide direct repeat (DR) that flanks the v-src gene in the Rous sarcoma virus.

Article Title: Determinants in the HTLV-1 Capsid Major Homology Region that are Critical for Virus Particle Assembly.
Article Snippet: The MHR region has been extensively studied among other different retroviruses, including HIV-1 [23-32], Rous sarcoma virus (RSV) [32-35], Mason-Pfizer monkey virus (MPMV) [36], and murine leukemia virus (MLV) [37].

Article Title: DHX15 inhibits mouse APOBEC3 deamination.
Article Snippet: It has recently been shown to play a role in viral genome packaging of Mason-Pfizer monkey https://doi.org/10.1371/journal.ppat.1013045.g004 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013045 April 1, 2025 10 / 18 virus through interaction with a glycine-rich region (G-patch) in Gag that is unique to this virus [56].

Article Title: Allostery and inter-domain dynamics in NXF1: An insight into viral CTE-RNA binding.
Article Snippet: Nucleocytoplasmic export of mRNA is a fundamental process in eukaryotic cells, facilitating the transportation of mRNA transcripts from nucleus to cytoplasm.. Central to this pathway is Nuclear Export Factor 1 (NXF1), a key RNA binding protein (RBP) mediating mRNA export through the Nuclear Pore Complex (NPC).. The significance of NXF1 in the export pathway extends to viral infections and neurodegenerative diseases where aberrations in nucleocytoplasmic transport have been identified as critical factors in disease progression.

RNA Binding Assay:

Article Title: Rev-RRE activity modulates HIV-1 replication and latency reactivation: Implications for viral persistence and cure strategies
Article Snippet: “Simple” retroviruses, such as the Mason-Pfizer Monkey Virus, contain an RNA structure in the IR-mRNAs, the CTE [ ], that directly recruits the cellular factors Nxf1 and Nxt1 to permit nuclear export.

Article Title: Unveiling the DHX15-G-patch interplay in retroviral RNA packaging.
Article Snippet: This study reveals a broad aspect of retroviral replication and highlights the critical role of the cellular DEAH box RNA helicase DHX15 in the life cycle of Mason-Pfizer monkey virus.

Article Title: Unveiling the DHX15-G-patch interplay in retroviral RNA packaging.
Article Snippet: Interestingly, Mason-Pfizer monkey virus (M-PMV), a member of the betaretroviral family, is unique among viruses by encoding a G-patch motif ( 18 ).

Article Title: DHX15 inhibits mouse APOBEC3 deamination
Article Snippet: It has recently been shown to play a role in viral genome packaging of Mason-Pfizer monkey virus through interaction with a glycine-rich region (G-patch) in Gag that is unique to this virus [ ].

Article Title: The snakehead retrovirus promoter functions independently of the 3'ORF protein and its products are maternally inherited in transgenic zebrafish.
Article Snippet: Examples include the constitutive transport element (CTE) of the Mason-Pfizer monkey virus (MPMV), which binds the NXF1(TAP)/ NXT1 cellular factors to enhance nuclear export of unspliced viral RNAs [32,33], or the 115-nucleotide direct repeat (DR) that flanks the v-src gene in the Rous sarcoma virus.

Article Title: Determinants in the HTLV-1 Capsid Major Homology Region that are Critical for Virus Particle Assembly.
Article Snippet: The MHR region has been extensively studied among other different retroviruses, including HIV-1 [23-32], Rous sarcoma virus (RSV) [32-35], Mason-Pfizer monkey virus (MPMV) [36], and murine leukemia virus (MLV) [37].

Article Title: DHX15 inhibits mouse APOBEC3 deamination.
Article Snippet: It has recently been shown to play a role in viral genome packaging of Mason-Pfizer monkey https://doi.org/10.1371/journal.ppat.1013045.g004 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013045 April 1, 2025 10 / 18 virus through interaction with a glycine-rich region (G-patch) in Gag that is unique to this virus [56].

Article Title: Allostery and inter-domain dynamics in NXF1: An insight into viral CTE-RNA binding.
Article Snippet: Nucleocytoplasmic export of mRNA is a fundamental process in eukaryotic cells, facilitating the transportation of mRNA transcripts from nucleus to cytoplasm.. Central to this pathway is Nuclear Export Factor 1 (NXF1), a key RNA binding protein (RBP) mediating mRNA export through the Nuclear Pore Complex (NPC).. The significance of NXF1 in the export pathway extends to viral infections and neurodegenerative diseases where aberrations in nucleocytoplasmic transport have been identified as critical factors in disease progression.

Reverse Transcription:

Article Title: Rev-RRE activity modulates HIV-1 replication and latency reactivation: Implications for viral persistence and cure strategies
Article Snippet: “Simple” retroviruses, such as the Mason-Pfizer Monkey Virus, contain an RNA structure in the IR-mRNAs, the CTE [ ], that directly recruits the cellular factors Nxf1 and Nxt1 to permit nuclear export.

Article Title: Unveiling the DHX15-G-patch interplay in retroviral RNA packaging.
Article Snippet: This study reveals a broad aspect of retroviral replication and highlights the critical role of the cellular DEAH box RNA helicase DHX15 in the life cycle of Mason-Pfizer monkey virus.

Article Title: Unveiling the DHX15-G-patch interplay in retroviral RNA packaging.
Article Snippet: Interestingly, Mason-Pfizer monkey virus (M-PMV), a member of the betaretroviral family, is unique among viruses by encoding a G-patch motif ( 18 ).

Article Title: DHX15 inhibits mouse APOBEC3 deamination
Article Snippet: It has recently been shown to play a role in viral genome packaging of Mason-Pfizer monkey virus through interaction with a glycine-rich region (G-patch) in Gag that is unique to this virus [ ].

Article Title: The snakehead retrovirus promoter functions independently of the 3'ORF protein and its products are maternally inherited in transgenic zebrafish.
Article Snippet: Examples include the constitutive transport element (CTE) of the Mason-Pfizer monkey virus (MPMV), which binds the NXF1(TAP)/ NXT1 cellular factors to enhance nuclear export of unspliced viral RNAs [32,33], or the 115-nucleotide direct repeat (DR) that flanks the v-src gene in the Rous sarcoma virus.

Article Title: Determinants in the HTLV-1 Capsid Major Homology Region that are Critical for Virus Particle Assembly.
Article Snippet: The MHR region has been extensively studied among other different retroviruses, including HIV-1 [23-32], Rous sarcoma virus (RSV) [32-35], Mason-Pfizer monkey virus (MPMV) [36], and murine leukemia virus (MLV) [37].

Article Title: DHX15 inhibits mouse APOBEC3 deamination.
Article Snippet: It has recently been shown to play a role in viral genome packaging of Mason-Pfizer monkey https://doi.org/10.1371/journal.ppat.1013045.g004 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013045 April 1, 2025 10 / 18 virus through interaction with a glycine-rich region (G-patch) in Gag that is unique to this virus [56].

Article Title: Allostery and inter-domain dynamics in NXF1: An insight into viral CTE-RNA binding.
Article Snippet: Nucleocytoplasmic export of mRNA is a fundamental process in eukaryotic cells, facilitating the transportation of mRNA transcripts from nucleus to cytoplasm.. Central to this pathway is Nuclear Export Factor 1 (NXF1), a key RNA binding protein (RBP) mediating mRNA export through the Nuclear Pore Complex (NPC).. The significance of NXF1 in the export pathway extends to viral infections and neurodegenerative diseases where aberrations in nucleocytoplasmic transport have been identified as critical factors in disease progression.



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SDS-PAGE and immunoblot analysis of cross-linked MA-CaM complexes . The mixture of CaM and myrMAPP as well as individual proteins were treated with DSBU and DSPU cross-linkers at increasing molar excesses of cross-linker to protein (5-fold molar excess and 10-fold molar excess) and analyzed by SDS-PAGE with Coomassie detection ( left panels ) or with immunoblot detection by the use of antibodies <t>against</t> <t>M-PMV</t> MA ( middle panels ) or against CaM ( right panels ). Individual proteins were detected as signals of ∼14.4 kDa (myrMAPP; illustrated by a gray circle ) and ∼17 kDa (CaM; illustrated by a blue square) which corresponds to their molecular weight (14.9 kDa of myrMAPP and 16.7 kDa of CaM). In mixed samples of myrMAPP and CaM, additional bands of higher molecular weight appear upon cross-linker treatment, indicating formation of oligomeric CaM–myrMAPP complexes. Data shown are representative of two independent biological replicates.
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SDS-PAGE and immunoblot analysis of cross-linked MA-CaM complexes . The mixture of CaM and myrMAPP as well as individual proteins were treated with DSBU and DSPU cross-linkers at increasing molar excesses of cross-linker to protein (5-fold molar excess and 10-fold molar excess) and analyzed by SDS-PAGE with Coomassie detection ( left panels ) or with immunoblot detection by the use of antibodies against M-PMV MA ( middle panels ) or against CaM ( right panels ). Individual proteins were detected as signals of ∼14.4 kDa (myrMAPP; illustrated by a gray circle ) and ∼17 kDa (CaM; illustrated by a blue square) which corresponds to their molecular weight (14.9 kDa of myrMAPP and 16.7 kDa of CaM). In mixed samples of myrMAPP and CaM, additional bands of higher molecular weight appear upon cross-linker treatment, indicating formation of oligomeric CaM–myrMAPP complexes. Data shown are representative of two independent biological replicates.

Journal: The Journal of Biological Chemistry

Article Title: Structural and functional insights into calmodulin-mediated lipid binding and proteolytic cleavage of the M-PMV matrix protein

doi: 10.1016/j.jbc.2025.111102

Figure Lengend Snippet: SDS-PAGE and immunoblot analysis of cross-linked MA-CaM complexes . The mixture of CaM and myrMAPP as well as individual proteins were treated with DSBU and DSPU cross-linkers at increasing molar excesses of cross-linker to protein (5-fold molar excess and 10-fold molar excess) and analyzed by SDS-PAGE with Coomassie detection ( left panels ) or with immunoblot detection by the use of antibodies against M-PMV MA ( middle panels ) or against CaM ( right panels ). Individual proteins were detected as signals of ∼14.4 kDa (myrMAPP; illustrated by a gray circle ) and ∼17 kDa (CaM; illustrated by a blue square) which corresponds to their molecular weight (14.9 kDa of myrMAPP and 16.7 kDa of CaM). In mixed samples of myrMAPP and CaM, additional bands of higher molecular weight appear upon cross-linker treatment, indicating formation of oligomeric CaM–myrMAPP complexes. Data shown are representative of two independent biological replicates.

Article Snippet: The matrix (MA) domain of the Mason-Pfizer monkey virus (M-PMV) Gag polyprotein plays a central role in retroviral assembly and trafficking, coordinating membrane association and proteolytic maturation.

Techniques: SDS Page, Western Blot, Molecular Weight

Co-immunoprecipitation of M-PMV Gag and HA-tagged CaM in HEK293T cells . HEK293T cells were co-transfected with plasmids encoding non-infectious M-PMV (pSARM4 RT) and HA-tagged human CaM (pCMV HA-CaM). Cell lysates were subjected to co-immunoprecipitation using anti-HA magnetic agarose beads (Chromotek HA-trap). Anti-MA antibody (1:2500) was used for immunoblot detection. The binding of HA-CaM was inspected by immunoblot using anti CaM antibody. Note, the bottom band is a nonspecific signal that was also detected in the mock sample of non-transfected cells (see ). Data shown are representative of three independent biological replicates.

Journal: The Journal of Biological Chemistry

Article Title: Structural and functional insights into calmodulin-mediated lipid binding and proteolytic cleavage of the M-PMV matrix protein

doi: 10.1016/j.jbc.2025.111102

Figure Lengend Snippet: Co-immunoprecipitation of M-PMV Gag and HA-tagged CaM in HEK293T cells . HEK293T cells were co-transfected with plasmids encoding non-infectious M-PMV (pSARM4 RT) and HA-tagged human CaM (pCMV HA-CaM). Cell lysates were subjected to co-immunoprecipitation using anti-HA magnetic agarose beads (Chromotek HA-trap). Anti-MA antibody (1:2500) was used for immunoblot detection. The binding of HA-CaM was inspected by immunoblot using anti CaM antibody. Note, the bottom band is a nonspecific signal that was also detected in the mock sample of non-transfected cells (see ). Data shown are representative of three independent biological replicates.

Article Snippet: The matrix (MA) domain of the Mason-Pfizer monkey virus (M-PMV) Gag polyprotein plays a central role in retroviral assembly and trafficking, coordinating membrane association and proteolytic maturation.

Techniques: Immunoprecipitation, Transfection, Western Blot, Binding Assay

Model of myrMAPP-CaM complex . A , structure of HIV-1 MA(8–43)-CaM complex published by Vlach et al . (PDB entry 2mgu) . CaM is colored in cyan , HIV-1 MA(8–43) in magenta and calcium ions in red . NTD – N-terminal domain, CTD – C-terminal domain. B , NMR chemical shift changes and differential HDX relative fractional uptake detected for CaM interacting with M-PMV myrMAPP mapped onto the published structure of free CaM and CaM in complex with HIV-1 MA(8–43) . Free CaM (PDB 1Cll) is shown on the left , and CaM, as observed in the complex with HIV-1 MA(8–43) on the right , both colored according to our M-PMV myrMAPP interaction results. Top panels : NMR chemical shifts, with residues showing shifts in orange and residues without detectable shifts in cyan . Bottom panels : Differential HDX relative fractional uptake, where differential deuterium uptake at 20 s is indicated by the intensity of blue coloring. C , structure of M-PMV MAPP ( gray ) in complex with CaM (cyan) calculated by HADDOCK with visualized identified cross-links between myrMAPP and CaM. Cross-links meeting the maximal distance of 35 Å are depicted as green dashes, cross-links exceeding the maximum distance of 35 Å are depicted as red dash. D , structure of M-PMV myrMAPP ( gray ) in complex with CaM ( cyan ) calculated by HADDOCK with visualized chemical shifts detected by NMR. Residues with chemical shifts detected in equimolar ratio of proteins in the analyzed myrMAPP sample are colored in orange , whereas those detected in the sample with increased concentration of CaM (1:5) are colored in blue , which also comprises the orange residues. E , structure of M-PMV MAPP in complex with CaM calculated by HADDOCK with visualized differential HDX relative fractional uptake. Degree of deuteration is shown as intensity of blue and red color. F , structure of M-PMV MAPP ( gray ) in complex with CaM ( cyan ) calculated by HADDOCK with visualized individual functional regions of MA. Residues in MA involved in MA oligomerization are colored in yellow ( , ), residues interacting with PI(4,5)P 2 are shown in magenta , residues interacting with the M-PMV cytotail are colored in green . Additionally, residue that interacts with both the M-PMV cytotail and PI(4,5)P 2 is colored blue and residue that interacts with the cytotail and also involved in oligomerization is colored in red .

Journal: The Journal of Biological Chemistry

Article Title: Structural and functional insights into calmodulin-mediated lipid binding and proteolytic cleavage of the M-PMV matrix protein

doi: 10.1016/j.jbc.2025.111102

Figure Lengend Snippet: Model of myrMAPP-CaM complex . A , structure of HIV-1 MA(8–43)-CaM complex published by Vlach et al . (PDB entry 2mgu) . CaM is colored in cyan , HIV-1 MA(8–43) in magenta and calcium ions in red . NTD – N-terminal domain, CTD – C-terminal domain. B , NMR chemical shift changes and differential HDX relative fractional uptake detected for CaM interacting with M-PMV myrMAPP mapped onto the published structure of free CaM and CaM in complex with HIV-1 MA(8–43) . Free CaM (PDB 1Cll) is shown on the left , and CaM, as observed in the complex with HIV-1 MA(8–43) on the right , both colored according to our M-PMV myrMAPP interaction results. Top panels : NMR chemical shifts, with residues showing shifts in orange and residues without detectable shifts in cyan . Bottom panels : Differential HDX relative fractional uptake, where differential deuterium uptake at 20 s is indicated by the intensity of blue coloring. C , structure of M-PMV MAPP ( gray ) in complex with CaM (cyan) calculated by HADDOCK with visualized identified cross-links between myrMAPP and CaM. Cross-links meeting the maximal distance of 35 Å are depicted as green dashes, cross-links exceeding the maximum distance of 35 Å are depicted as red dash. D , structure of M-PMV myrMAPP ( gray ) in complex with CaM ( cyan ) calculated by HADDOCK with visualized chemical shifts detected by NMR. Residues with chemical shifts detected in equimolar ratio of proteins in the analyzed myrMAPP sample are colored in orange , whereas those detected in the sample with increased concentration of CaM (1:5) are colored in blue , which also comprises the orange residues. E , structure of M-PMV MAPP in complex with CaM calculated by HADDOCK with visualized differential HDX relative fractional uptake. Degree of deuteration is shown as intensity of blue and red color. F , structure of M-PMV MAPP ( gray ) in complex with CaM ( cyan ) calculated by HADDOCK with visualized individual functional regions of MA. Residues in MA involved in MA oligomerization are colored in yellow ( , ), residues interacting with PI(4,5)P 2 are shown in magenta , residues interacting with the M-PMV cytotail are colored in green . Additionally, residue that interacts with both the M-PMV cytotail and PI(4,5)P 2 is colored blue and residue that interacts with the cytotail and also involved in oligomerization is colored in red .

Article Snippet: The matrix (MA) domain of the Mason-Pfizer monkey virus (M-PMV) Gag polyprotein plays a central role in retroviral assembly and trafficking, coordinating membrane association and proteolytic maturation.

Techniques: Concentration Assay, Functional Assay, Residue